![]() Even if you did have the time, you still wouldn’t have the energy. The harder you try to get more out of your time, it seems, the less time you have. You would start outsourcing some of the load, but you’re so busy and so exhausted, you can’t even explain what’s keeping you so busy. With each new tactic you learn, each new “life hack,” each new shortcut, life gets more hectic. Your anxiety levels are sky-high, and you’re becoming forgetful. It seems there’s not enough coffee in the world to keep you going. You can steal a couple extra hours of work on your laptop after everyone in the house has gone to bed. You used to sleep eight hours a night, but now you’ll sleep five. You start searching for extra bits of time, like loose change under couch cushions. You’re holding conference calls while driving to work. You’re checking your email while brushing your teeth. Next, you figure, you can get more out of your time if you do two things at once. No more lunches with your friends – you’ll eat at your desk. You start cutting out all but the most essential activities that move you toward your goals. You start “speed reading,” and listening to audiobooks and podcasts on 3x speed. Your calendar becomes jam-packed with a kaleidoscope of colored blocks. You may even start saying “no” to some opportunities, so you can make better use of your time.īut it becomes harder and harder to get more out of your time. You’re learning keyboard shortcuts for the apps you use every day. You’re building routines, so you can get repeating tasks done faster. You’re keeping a calendar, so you don’t forget things. When you start managing your time, you find you really are getting more done. Time management, it seems, is critically important. “There’s only twenty-four hours in a day.” The natural conclusion we’re supposed to draw from this common observation is: If there are only so many hours in a day, you should make the most of each of those precious hours.
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![]() Then double click on the Minecraft file and it will begin to install Minecraft on your computer. We suggest moving that "Minecraft.exe" file from your downloads folder to your Desktop. Make sure to click on the "Microsoft.exe" for the Windows 7/8 (alternative) otherwise it will not work correctly.Īs soon as you click on that icon it will download a file to your computer. exe version in order to play Minecraft on a school computer.Ĭurrently the ONLY version that works on school computers is the “Alternate Version” for Windows 7/8 made available at /en-us/download/alternative The image below shows the specific. School policy prevents students from having admin rights. msi, one must have administrative rights on their computers. Also support from our HelpDesk will be limited due to these system requirement issues.Īlso students cannotinstall Minecraft using the. Therefore students attempting to play Minecraft on a school computer should operate at their own risk and understand that game performance may not be the best. Most of our computers meet the minimum system requirements. In particular the graphics card series and processor speed are below the recommended system requirements. Its recommended system requirements do not match the hardware we have in many of our school computers. Minecraft requires some specific hardware to run. Other activities in the game include exploration, resource gathering, crafting, and combat. If you have a lot of worlds and do not want to search through all the folders to locate the world file you want, sort the minecraftWorlds folder by date and open the folder with a date/time close to the last time you were in the world.Minecraft is a computer game in which players to build constructions out of textured cubes in a 3D procedurally generated world. Result: The text file opens and displays the world name as it appears in Minecraft Education.
![]() ![]() ![]() ![]() Upon finishing the installation, you will finally be able to load the app. After downloading it to your local storage, to access the app all you need to do is to follow the simple on-screen instructions. Since ZBrush has experienced a constant flow of upgrades and redesigns since it appeared first on the market in 1999, the latest version of this app comes inside a significantly large installer package. The only limitation it has is that it cannot be used for commercial or for-profit purposes. Today, ZBrush 2022 is commonly used in creating movie/VFX projects, video games, illustrations, advertising, scientific visualization, 3D printing, Automotive, and industrial design, jewelry design, education, and much more.Ī free version of the app comes in the form of a 45-day trial with a fully unlocked toolset that professional users are using to create their masterpieces. ZBrush 2022 provides enhancements that affect many areas of your workflow, with a focus on innovative new features that are designed to fuel exploration and inspire creativity.īuilt from the ground up to promote creativity and a natural style of painting, It has evolved a lot in the past decades, becoming one of the most commonly used modeling apps for personal users, students, professionals, and large organizations. ![]() ![]() ![]() While the binding preferences between standard nucleobases and amino acids have in general been well studied 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, much less is known about how these preferences change in the case of typical nucleobase modifications, with most work focusing on the overall impact of modifications on the affinities between complete nucleic acids and proteins 21, 22, 23, 24. Importantly, such recognition also directly depends on the intrinsic binding preferences between the individual nucleobases and amino acids in different environments 11. In general, DNA and RNA recognition by proteins depends on different environmental, structural and dynamical determinants. However, less is known about the atomistic mechanisms behind such phenotypic changes and, in particular, how nucleobase modifications affect the interactions between the modified nucleic acids and protein readers, which detect them. Notably, the impact of the DNA/RNA modifications on gene expression and transcript stability has mostly been studied from the perspective of how the change in their genomic or transcriptomic patterns affects the cellular or organismic phenotype 3, 5. In RNA, nucleobase modifications affect different molecular processes, including mRNA transcription, splicing, export, translation and degradation 3, 7, 8, 9, 10. In DNA, modified nucleobases play key roles in cell differentiation, aging and disease development by either remodeling chromatin structure or affecting directly the DNA/protein interactions 1, 2, 4, 5, 6. Modifications of standard DNA/RNA nucleobases greatly amplify the amount of information encoded in nucleic-acid sequences and are associated with epigenetic regulation of gene expression and modulation of transcript stability 1, 2, 3. Our results contribute towards establishing a quantitative foundation for understanding, predicting and modulating the interactions between modified nucleic acids and proteins at the atomistic level. For those two modified bases, the effect is more nuanced and manifests itself primarily at the level of absolute changes in the binding free energy. Conversely, we show that the relative ordering of sidechain affinities for 5hmC and m 6A does not differ significantly from those for their precursor bases, cytosine and adenine, respectively, especially in the low dielectric environment. Our analysis identifies protein side chains that are highly tuned for detecting cytosine methylation as a function of the environment and can thus serve as microscopic readers of epigenetic marks. We verify the derived affinities by comparing against a comprehensive set of high-resolution structures of nucleic-protein complexes involving 5mC. We derive here the absolute binding free energies and analyze the binding modalities between key modified nucleobases 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and N 6-methyladenine (m 6A) and all non-prolyl/non-glycyl protein side chains using molecular dynamics simulations and umbrella sampling in both water and methanol, the latter mimicking the low dielectric environment at the dehydrated nucleic-acid/protein interfaces. We conjecture that this thermal hierarchy reflects an underlying temporal order, and that side-chain ordering facilitates the search for the correct backbone topology.Covalent modifications of standard DNA/RNA nucleobases affect epigenetic regulation of gene expression by modulating interactions between nucleic acids and protein readers. Our results indicate a thermal hierarchy of ordering events, with side-chain ordering appearing at temperatures below the helix-coil transition but above the folding transition. ![]() ![]() Rather, side-chain ordering is spread over a wide temperature range. B, 111 (2007) 4244) we do not find collective effects leading to a separate transition. In contrast to earlier studies on homopolypeptides (Wei et al., J. Concepts of circular statistics are introduced to analyze side-chain fluctuations. For this purpos e we have performed multicanonical simulations of the villin headpiece subdomain HP-36, an often used to y model in protein studies. Wei and 2 other authors Download PDF Abstract: We investigate the relation between backbone and side-chain ordering in a small protein. Download a PDF of the paper titled Backbone and Sidechain Ordering in a small Protein, by Y. ![]() ![]() ![]() It is a decentralized payment network that allows for instant, secure, and low-cost payments. Ripple is a cryptocurrency xrp created in 2012. Coinbase halted XRP trading on January 19. According to a January 19 SEC filing, Ripple Labs was accused of illegally profiting from more than $1 billion in profits by offering digital-asset securities that were not registered with the SEC. XRP cannot be purchased directly from Coinbase at this time. Can Us Residents Trade Xrp?ĭue to SEC actions, XRP trading for U.S. Furthermore, the company has two Payment institution licenses in the EU, as well as a BitLicense in New York, making it one of the most secure exchanges. ![]() Bitstamp Is A Secure And Popular Cryptocurrency Exchangeīitstamp, one of the world’s leading cryptocurrency exchanges, operates in a number of countries around the world. XRP is the native token of the Ripple network, a distributed ledger platform that facilitates cross-border payments. Bitstamp is a global cryptocurrency exchange, providing access to trading services in over 50 countries. Yes, you can buy XRP on Bitstamp in New York. Many people who have sold XRP believe they did nothing wrong, and that the SEC opinion is unfair. exchanges have temporarily halted XRP trading as a result of the opinion. In a written opinion, the SEC Division of Corporation Finance declares that “everyone who sold XRP – including you and me – is engaging in illegal activity.” According to the Division’s opinion, XRP is a security and that “all XRP sales were illegal.” Only Ripple and its affiliates are not shown in the opinion to have illegally sold XRP. The SEC lawyer could not provide any such evidence, so the judge asked for a written opinion from the SECs Division of Corporation Finance on the matter. The SEC attorney was not convinced, and the judge demanded that he provide evidence that only Ripple and affiliates of Ripple could have sold XRP illegally. It is possible for an XRP seller to only sell to family or friends in person. The seller will need to purchase multiple components when selling XRP in person, including an XRP wallet loaded with the amount he wants to sell. The in-person P2P transaction is more private because it does not involve third-party intermediaries. Most DEXs created with non-XRPL blockchains do not support XRP. In most cases, users do not need to register with a centralized account instead, they can connect to a wallet that works with them. A decentralized exchange (DEX) connects buyers and sellers online without the involvement of a third party. Some exchanges have limits on how much money a new user can trade on. You may want to consider hiring an OTC trading desk if you intend to sell a large amount (up to $1,000,000). Most of the time, when selling XRP on a centralized crypto exchange, you must follow a few simple steps. ![]() There are numerous ways to sell XRP for USD. To sell XRP, you may need to know how to send coins from your wallet or account to the storage place (typically XRP). It is important to research different options and compare prices before making a decision.Ī blockchain, also known as the XRP Ledger or XRPL, is a type of digital asset. Some people may choose to sell their XRP on online exchanges, while others may opt to use a peer-to-peer platform. There is no one definitive answer to this question. Binance, on the other hand, does not have a minimum deposit or high fees, whereas Kraken does not. Coinbase is a high-level digital currency exchange in the United States that offers access to thousands of coins. When you sign up for eToro, you can purchase XRP in the United States in as little as five minutes. Mobile apps for iOS and Android are available through the company’s or mobile site. Coinbase is only accessible through a bank account or debit card. Users of New York City can now purchase XRP, the third most popular cryptocurrency in terms of volume, from the city. In this article, we’ll take a look at some of the best ways to trade Ripple in New York. There are a number of different exchanges that offer XRP trading, and each has its own advantages and disadvantages. New York is one of the world’s financial hubs, so it’s no surprise that there is significant interest in trading Ripple in the city. For example, Ripple is faster and more scalable than Bitcoin, making it more suitable for large-scale payments. One of the most popular cryptocurrencies is Ripple (XRP), which offers a number of advantages over other digital currencies. Cryptocurrency trading is becoming increasingly popular, as investors look to take advantage of volatile markets. ![]() ![]() ![]() Rowling, Kuroshitsuji | Black ButlerĪfter Harry's vacation death and his sisters sent Harry on a trip to another universe. Language: English Words: 12,193 Chapters: 4/? Kudos: 22 Bookmarks: 2 Hits: 211 He could have given up on her once he found out she was more than he had bargained for, but the risk only makes the reward that much sweeter. Sebastian Michaelis is a man with several strange habits. One catches the eye of a man who wants to sink his teeth into her, another may save her life. Grelle Sutcliffe is a woman of many talents. Pigeonfisch Fandoms: Kuroshitsuji | Black Butler Language: English Words: 3,240 Chapters: 1/? Kudos: 7 Bookmarks: 1 Hits: 31 It was the fault of a twisted man who made the choice to target a helpless child. He was trying to cope with a trauma that nobody, let alone a child, should have to experience. He is a broken child who’s struggling to cope with the loss of his innocence which had been stripped away from him much too young. She couldn’t help but feel frustrated and almost defeated but she knew it wasn’t Sirius’ fault. But also you probably shouldn’t touch him.I’m sorry Vincent but I needed a villian.Ciel Phantomhive & Real Ciel Phantomhive.Creator Chose Not To Use Archive Warnings.Sirxiuss Fandoms: Kuroshitsuji | Black Butler Language: English Words: 4,353 Chapters: 1/? Comments: 4 Kudos: 12 Bookmarks: 3 Hits: 129 “Because he isn’t human,” she said, giving them a patronizingly sweet smile, “he hasn’t taken a single breath since you got here.” Series If she was being honest with herself, she had to admit, she was glad he thought her interest in the butler was carnal-it made the look on his face that much more entertaining. “And why, precisely, would you be interested in him?” The kid asked, boredom and exasperation clear in his tone. He was conventionally handsome-tall, clear skin, perfectly white teeth, raven-dark hair, piercing maroon eyes, and a disarming smile to match. She figured that people must ask about his butler often. That statement only seemed to annoy the kid further, his eyes moving slightly-as if he were fighting the urge to roll them-before resuming their place on her. “First, I’d like to know more about that butler of yours.” The subtle, yet persistent, feeling of being out of place. but she actually gets to be badass on paper.Ciel Phantomhive & Original Female Character(s).Sebastian Michaelis/Original Female Character(s).Now that his whole world is turned upside down, what will Sebastian Michaelis do? Language: English Words: 5,705 Chapters: 4/? Comments: 1 Kudos: 50 Bookmarks: 8 Hits: 699ĪDHD_Enthusiast Fandoms: Kuroshitsuji | Black Butler The balance between executing his master's absolute orders, his contract for his master's soul and his upkeep with butler aesthetics gets severely torn apart when one simple word escapes Ciel's lips. She makes a deal with a demon in exchange for her soul, unaware he desires more than just her soul at the end of their arrangement.īut what will this demon do when feelings get involved? Language: English Words: 52,812 Chapters: 15/? Comments: 41 Kudos: 211 Bookmarks: 48 Hits: 4,837ĠTheDuchess0 Fandoms: Kuroshitsuji | Black Butlerįrom the realm of the heartless, emotionless predators known as demons comes a particular figure who temporarily goes by the name of Sebastian Michaelis. Noah no Hakobune Circus | Noah's Arc CircusĬelia Phantomhive loses everything but the all-consuming need for revenge. ![]() Angelina Dalles| Madame Red & Grell Sutcliff.Doughnut_mind_if_I_do Fandoms: Kuroshitsuji | Black Butler ![]() ![]() ![]() MoreĬHINA LAUNCHES NEW BEIDOU SATELLITES, ROCKET BOOSTER LANDS NEAR HOUSE - China added a new pair of satellites to its Beidou positioning and navigation system late Monday, but spent stages from the launch landed within inhabited areas.Ī Long March 3B equipped with a Yuanzheng-1 upper stage lifted off from the Xichang Satellite Launch Center at 10:26 p.m. Similar to SpaceX's Starlink, Project Kuiper is Amazon's plan to provide high-speed internet by launching and connecting 3,236 satellites in low Earth orbit (. PROJECT KUIPER: AMAZON'S ANSWER TO SPACEX'S STARLINK PASSES 'CRUCIAL' TEST - Amazon's upcoming satellite broadband network, dubbed "Project Kuiper," just passed a key test test that paves the way for a 2024 launch. The Taiyuan Satellite Launch Center launched the rocket from waters off the coast of Yangjiang, south China's Guangdong Province, at 6:39 a.m. MoreĬHINA LAUNCHES 3 SATELLITES FROM SEA - China on Tuesday successfully launched a Long March-11 carrier rocket into space, sending three satellites into planned orbit. This particular booster, tail number B1058, was coming back from its record-breaking 19th missio. During its return voyage to Port Canaveral in Central Florida, a SpaceX Falcon 9 first stage booster toppled over and broke in half. HISTORIC SPACEX FALCON 9 BOOSTER TOPPLES OVER AND IS LOST AT SEA - A piece of America’s space history is now on the ocean’s floor. The space agency shared a new video showcasing its 2023 achievements, ranging from the successful return of its first asteroid sample. NASA HAILS HISTORIC SPACE MILESTONES OF 2023 IN INSPIRING NEW VIDEO - t has been a busy year for NASA with the agency's solid list of historic missions and inspiring milestones that continue to push the limits of human exploration. ![]() The Alpha rocket lifted off from Vandenberg Space Force Base in California at 12:32 p.m. MoreįIREFLY ALPHA UPPER STAGE MALFUNCTION PUTS PAYLOAD INTO WRONG ORBIT - A problem with the upper stage of a Firefly Aerospace Alpha rocket placed a Lockheed Martin technology demonstration satellite into the wrong orbit on a Dec. RUSSIA EXTENDS CROSS-FLIGHT PROGRAMME WITH NASA UNTIL 2025 - ROSCOSMOS - Russia's space state agency Roscosmos said on Thursday that a cross-flight programme with NASA to the International Space Station (ISS) had been extended until 2025, Interfax news agency reported. This mission comes on the heels of the Falcon Heavy launch, sending the X-37B milit. A Falcon 9 rocket launched the Starlink 6-36 mission from Cape Canaveral Space Force Station at 11:01 p.m. SPACEX ACES MODERN-DAY LAUNCH TURNAROUND RECORD WITH FALCON 9 STARLINK MISSION - SpaceX completed double-header launch night as the end of the year approaches. EST (0107 UTC) on Thursday at the opening of a ten-minute launch window. Liftoff of the USSF-52 mission from NASA’s Kennedy Space Center occurred at 8:07 p.m. SPACEX LAUNCHES FALCON HEAVY ROCKET, X-37B MILITARY SPACEPLANE - The second time was the charm for SpaceX as it was able to launch the X-37B military spaceplane onboard its Falcon Heavy rocket. India is set to launch its first X-Ray Polarimeter Satelli. ISRO TO WELCOME 2024 WITH INDIA’S FIRST X-RAY POLARIMETER SATELLITE LAUNCH ON JAN 1 - ISRO’s XPoSat mission will help understand emission mechanisms of various astronomical sources like black holes, neutron stars.īuilding on the momentum of 2023, Indian Space Research Organisation or ISRO is set to start 2024 with a bang. ![]() ![]() ![]() To hit 25 players with the Earth Shock Ability, it’s better to sneak up and catch them unaware or use the ability as an opener to the battle. You are also awarded no invincibility frames while using the ability, which means you will often die between pressing and connecting the ability with the opponent on the other side. The Brujah Vandal’s Earth Shock ability is supposed to stun an enemy however, you will quickly notice that all it really does is push them back a little and blocks them from doing anything for what seems like 0.5 seconds or less, which makes this ability unviable as an offensive skill.
![]() ![]() This can take a lot more work and coordination, but may make sense for highly collaborative and large manuscripts.ĭecide with your PI who will be corresponding author. An alternative is to generate the initial outline or first draft with the help of co-authors. “Equal contribution” authorship positions for the first and last authors is a newer option for a number of journals. (This is helpful to do in any case as many journals now require this information.) Assigning authorship is sometimes complicated, but keep in mind that the Acknowledgements can be used to recognize those who made minor contributions (including reading the manuscript to provide feedback). If the study involved many people, create a table of possible authors showing their specific contributions to the manuscript. ![]() ![]() Make sure the manuscript conforms to the target journal’s word and figure limits.ĭiscuss all possible authors with your PI. Write your abstract after the first draft is completed. Don’t focus on writing perfect prose for the first draft. Decide which article type you would like to submit and reformat your outline according to the journal’s standards (including citation style).Ĭonvert your outline (including the figure captions) to complete sentences. Once you’ve chosen your journal, look at the journal’s article types. I also like to vary which journals I publish in, both to broaden the potential readers of my papers and to avoid the appearance of having an unfair “inside connection” to a given journal. I like to consider my ideal target audience. ![]() You first need to decide where you want to submit your manuscript. If not, you should have a good idea of what it will take to finish the manuscript. Is it a complete story? If so, you’re ready to prepare for submission. Now read the entire outline (including the figures). It is important to include a bullet describing the limitations of the presented work. These bullets should refer to your figures.ĭiscussion: Summarize your findings in the context of prior work. Results: What were your findings? Each major finding should be its own bullet, with sub-bullets going into more detail for each major finding. Important methodologies and materials, i.e., blinding for subjective analyses, full names of cell lines/strains/reagents and your commercial/academic sources for them. If you used animal or human subjects, include a bullet on ethics approval. Methods: What techniques did you use? Each technique should be its own bullet, with sub-bullets for key details. Introduction: What did you study, and why is it important? What is your hypothesis/research question? Here’s what should go in each of these sections: Google Docs can be helpful for maintaining a single version of the manuscript, but citation software often doesn’t play well with Google Docs (whereas most software options can automatically update citation changes in Word). Decide from the beginning what word processing software you’ll use (Word, Google Docs, etc.). Use Endnote, Reference Manager, Mendeley, or other citation software to start inserting references to go with bullets. This depends on the journal, but typically, and with minor variations: Introduction, Methods, Results, Discussion. Generate a bulleted outline of the major points for each section of the manuscript. Write bulleted captions for the figures/tables, including a title that explains the key finding for each figure/table, an explanation of experimental groups and associated symbols/labels, and details on biological and technical replicates and statements (such as “one of four representative experiments are shown.”) This will form the basis of your Results section. Gather your proposed figures and tables in a sequence that tells a story. Everything else will be spun around this. Write down your hypothesis/research question. Involve your principal investigator (PI) early and throughout the process. Here’s some thoughts on how to approach writing manuscripts based on original biomedical research. It’s hard work, but planning and organization helps. I’ve had the privilege of writing a few manuscripts in my research career to date, and helping trainees write them. ![]() |
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